2382625ccdfbedeb36dd71edb478dc81a6294ad

In the pipeline amgen

In the pipeline amgen your

The significant differences between the groups were examined by one-way analysis of variance (ANOVA) with the least significant difference test. Values of pThree representative compounds and four active ingredients in NTF had been verified respectively by HPLC and HRMS, which are shown in Figure 2. The in the pipeline amgen compounds were quantified: ligustrazine hydrochloride 2.

The prominent ions mass spectra of the fragment ions of the four active components were as follows: bassianin (compound 1) 114. The above analysis and the standard chemical structures of compounds 1, 2, 3 and 4 showed that the NTF extracts contained bassianin, cholesteryl ferulate, hyrcanoside and (4E,6E,2S,3R)-2-N-docosanoyl-4,6-tetradecasphingadienine.

Figure 2 Chemical ingredients analysis of NTF. Representative ingredients of NTF (A) and standards (B). The chromatogram, mass spectrum and structural formula of four compounds: (C) Compound 1; and health Compound 2; (E) Compound 3; (F) Compound 4.

Predictions of NTF on ischemic stroke and CIRI were investigated by network analysis as shown in Figure 3. In the network, the size of node was positively correlated with its degree.

In order to clarify the relationship between the herbs and potential active compounds, the herb-compound network of NTF is constructed in Figure 3A, from which we could find out that the FA (MOL000433), cholesteryl ferulate, etc. In this process we conducted target fishing on the 38 candidate active compounds which the 4 herbs yielded, obtaining 660 potential related targets after eliminating the duplicates. Meanwhile the targets about CIRI were collected from the integration of GeneCards and OMIM databases.

In the end, 2849 human targets were identified as being associated with the pathological mechanism of ischemic stroke and CIRI after eliminating the redundancy. Further analysis revealed that 367 targets were shared between 660 combined targets and 2849 in the pipeline amgen targets in Figure 3B.

There were 367 nodes and 2293 edges in total. The topological feature analysis of the PPI network was based on three major parameters of DC, BC and CC which were calculated by the CytoNCA plug-in for Cytoscape 3.

According to the related potential active components, we constructed the herb-compound-target network based on 43 key targets (Figure 3E). In this network, the top seven in the pipeline amgen were ecdysterone, which holds relevancy to 14 key targets; bassianin, which held relevancy to 13 key targets; cholesteryl ferulate, which was related to 12 key targets; beauvericin, related to 11 key targets; hyrcanoside, ergotamine and lupeol acetate were associated with 10 key targets.

Table 2 Information on 43 Hub TargetsFigure 3 Prediction results of network pharmacology of NTF on ischemic stroke and CIRI. Green nodes represent the herbs of NTF, orange nodes represent the Polifeprosan 20 with Carmustine (Gliadel)- FDA compounds of NTF, and blue nodes represent the other active compounds of NTF. The node color changes from yellow to red reflect the degree centrality changes from low to high.

The potential therapeutic target network of NTF is presented in Figure 4. We found that the top ten biological processes terms (ppp Figure 4 Functional analysis of NTF. We conducted KEGG pathway enrichment analysis on 43 target genes and screened out 14 in the pipeline amgen based on the threshold of pJudging from the in the pipeline amgen analysis of in the pipeline amgen key targets network, PIK3CA had higher DC, BC and CC than other protein targets.

According to the docking score whose absolute value was greater in the pipeline amgen 5, we selected 3 common hub compounds, which were the in the pipeline amgen, bassianin and cholesteryl ferulate against the active sites of the identified protein targets PIK3CA.

The total 2D and 3D interaction diagrams are respectively shown in Figure 5. Figure 5 3D and 2D interaction diagrams of hyrcanoside (Aa), bassianin (Bb) and cholesteryl ferulate (Cc) in the active site of PIK3CA (PDB ID 4TUU).

To further observe the effect of pretreatment with NTF extract on the degree of injury in rats, a neurological scores system was first established to evaluate scathing degree based on behavioral changes in rats after 24 h of reperfusion.

TTC staining was used to evaluate cerebral infarct volume.

Further...

Comments:

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